Target：NF-YB

Fields：Antigen processing and presentation;Tuberculosis;Human T-cell leukemia virus 1 infection

Gene Name：NFYB

Protein Name：Nuclear transcription factor Y subunit beta

Human Gene Id：4801

Human Swiss Prot No：P25208

Mouse Gene Id：18045

Mouse Swiss Prot No：P63139

Rat Gene Id：25336

Rat Swiss Prot No：P63140

Immunogen：The antiserum was produced against synthesized peptide derived from human NFYB. AA range:1-50

Specificity：NF-YB Polyclonal Antibody detects endogenous levels of NF-YB protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：NFYB;HAP3;Nuclear transcription factor Y subunit beta;CAAT box DNA-binding protein subunit B;Nuclear transcription factor Y subunit B;NF-YB

Observed Band(KD)：29kD

Background： The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008],

Function：domain:Can be divided into 3 domains: the weakly conserved A domain, the highly conserved B domain thought to be involved in subunit interaction and DNA binding, and the Glu-rich C domain.,function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYB/HAP3 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.,

Subcellular Location：Nucleus.

Expression： Urinary bladder,