Target：NB5R2

Fields：Amino sugar and nucleotide sugar metabolism

Gene Name：CYB5R2

Protein Name：NADH-cytochrome b5 reductase 2 (b5R.2) (EC 1.6.2.2)

Human Gene Id：51700

Human Swiss Prot No：Q6BCY4

Mouse Swiss Prot No：Q3KNK3

Rat Swiss Prot No：Q6AY12

Immunogen：Synthesized peptide derived from human protein . at AA range: 120-200

Specificity：NB5R2 Polyclonal Antibody detects endogenous levels of protein.

Formulation：Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500-2000 ELISA 1:5000-20000

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Observed Band(KD)：30kD

Background： The protein encoded by this gene belongs to the flavoprotein pyridine nucleotide cytochrome reductase family of proteins. Cytochrome b-type NAD(P)H oxidoreductases are implicated in many processes including cholesterol biosynthesis, fatty acid desaturation and elongation, and respiratory burst in neutrophils and macrophages. Cytochrome b5 reductases have soluble and membrane-bound forms that are the product of alternative splicing. In animal cells, the membrane-bound form binds to the endoplasmic reticulum, where it is a member of a fatty acid desaturation complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014],

Function：catalytic activity:NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5.,cofactor:FAD.,function:NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).,similarity:Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.,similarity:Contains 1 FAD-binding FR-type domain.,tissue specificity:Restricted expression.,

Subcellular Location：nucleus,mitochondrion,endoplasmic reticulum membrane,membrane,integral component of membrane,

Expression：Restricted expression.