Target：Rho H

Fields：Leukocyte transendothelial migration;Salmonella infection

Gene Name：RhoH

Protein Name：Rho-related GTP-binding protein RhoH

Human Gene Id：399

Human Swiss Prot No：Q15669

Mouse Gene Id：74734

Mouse Swiss Prot No：Q9D3G9

Immunogen：The antiserum was produced against synthesized peptide derived from human RhoH. AA range:141-190

Specificity：Rho H Polyclonal Antibody detects endogenous levels of Rho H protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RHOH;ARHH;TTF;Rho-related GTP-binding protein RhoH;GTP-binding protein TTF;Translocation three four protein

Observed Band(KD)：21kD

Background： The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013],

Function：disease:A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.,similarity:Belongs to the small GTPase superfamily. Rho family.,tissue specificity:Transcribed only in hemopoietic cells.,

Subcellular Location：Cytoplasm . Cell membrane ; Lipid-anchor ; Cytoplasmic side . Colocalizes together with ZAP70 in the immunological synapse. .

Expression：Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.