Target：PRIM1

Fields：DNA replication

Gene Name：PRIM1

Protein Name：DNA primase small subunit

Human Gene Id：5557

Human Swiss Prot No：P49642

Mouse Gene Id：19075

Mouse Swiss Prot No：P20664

Immunogen：The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420

Specificity：PRIM1 Polyclonal Antibody detects endogenous levels of PRIM1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PRIM1;DNA primase small subunit;DNA primase 49 kDa subunit;p49

Observed Band(KD)：50kD

Background： The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008],

Function：function:DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.,miscellaneous:The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA.,similarity:Belongs to the eukaryotic-type primase small subunit family.,subunit:Heterodimer of a small subunit and a large subunit.,

Subcellular Location：nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex,

Expression： Bone marrow,Thymus,