Target：PP2AA

Fields：mRNA surveillance pathway;Sphingolipid signaling pathway;Oocyte meiosis;Autophagy - other;Autophagy - animal;PI3K-Akt signaling pathway;AMPK signaling pathway;Adrenergic signaling in cardiomyocytes;TGF-beta signaling pathway;Hippo signaling pathway;Tight junction;Dopaminergic synapse;Long-term depression;Chagas disease;Hepatitis C;Human papillomavirus infection

Gene Name：PPP2CA

Protein Name：Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Human Gene Id：5515

Human Swiss Prot No：P67775

Mouse Gene Id：19052

Mouse Swiss Prot No：P63330

Rat Gene Id：24672

Rat Swiss Prot No：P63331

Immunogen：The antiserum was produced against synthesized peptide derived from human PP2A-alpha. AA range:260-309

Specificity：PP2A-Cα Polyclonal Antibody detects endogenous levels of PP2A-Cα protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PPP2CA;Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;Replication protein C;RP-C

Observed Band(KD)：35kD

Background： This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],

Function：catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation

Subcellular Location：Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .

Expression： Fibroblast,Liver,Lung,Placenta,Testis,Uterus,