Target:PUMA
Fields:Platinum drug resistance;p53 signaling pathway;Apoptosis;Apoptosis - multiple species;Hippo signaling pathway;Huntington disease;Measles;Pathways in cancer;Colorectal cancer
Gene Name:BBC3
Protein Name:Bcl-2-binding component 3
Human Gene Id:27113
Human Swiss Prot No:Q9BXH1
Mouse Gene Id:170770
Mouse Swiss Prot No:Q99ML1
Rat Gene Id:317673
Rat Swiss Prot No:Q80ZG6
Immunogen:The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169
Specificity:PUMA Polyclonal Antibody detects endogenous levels of PUMA protein.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:BBC3;PUMA;Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis
Observed Band(KD):23kD
Background: This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011],
Function:function:Essential mediator of p53-dependent and p53-independent apoptosis.,induction:By DNA damage, glucocorticoid treatment, growth factor deprivation and p53.,similarity:Belongs to the Bcl-2 family.,subcellular location:Localized to the mitochondria in order to induce cytochrome c release.,subunit:Interacts with MCL1 and BCL2A1 (By similarity). Interacts with BCL2 and BCL2L1/BCL-XL.,tissue specificity:Ubiquitously expressed.,
Subcellular Location:Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release.
Expression:Ubiquitously expressed.