Target：PU.1

Fields：Osteoclast differentiation;Human T-cell leukemia virus 1 infection;Pathways in cancer;Transcriptional misregulation in cancer;Acute myeloid leukemia

Gene Name：SPI1

Protein Name：Transcription factor PU.1

Human Gene Id：6688

Human Swiss Prot No：P17947

Mouse Gene Id：20375

Mouse Swiss Prot No：P17433

Rat Gene Id：366126

Rat Swiss Prot No：Q6BDS1

Immunogen：The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230

Specificity：PU.1 Polyclonal Antibody detects endogenous levels of PU.1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：SPI1;Transcription factor PU.1;31 kDa-transforming protein

Observed Band(KD)：32kD

Background： This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Function：function:Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.,induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of SFFV.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds DNA as a monomer. Interacts with RUNX1 and SPIB. Interacts with CEBPD and NONO.,

Subcellular Location：Nucleus .