Target：TRAC-1

Fields：RIG-I-like receptor signaling pathway

Gene Name：RNF125

Protein Name：E3 ubiquitin-protein ligase RNF125

Human Gene Id：54941

Human Swiss Prot No：Q96EQ8

Mouse Gene Id：67664

Mouse Swiss Prot No：Q9D9R0

Immunogen：The antiserum was produced against synthesized peptide derived from human RNF125. AA range:131-180

Specificity：TRAC-1 Polyclonal Antibody detects endogenous levels of TRAC-1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：RNF125;E3 ubiquitin-protein ligase RNF125;RING finger protein 125;T-cell RING activation protein 1;TRAC-1

Observed Band(KD)：26kD

Background：ring finger protein 125(RNF125) Homo sapiens This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012],

Function：function:E3 ubiquitin-protein ligase that acts as a positive regulator of T-cell activation. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,tissue specificity:Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4+ and CD8+ T-cells, suggesting that it is preferentially confined to T-cells.,

Subcellular Location：Golgi apparatus membrane ; Lipid-anchor . Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane. .

Expression：Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells.