Target：Topo IIIβ-1

Fields：Homologous recombination;Fanconi anemia pathway

Gene Name：TOP3B

Protein Name：DNA topoisomerase 3-beta-1

Human Gene Id：8940

Human Swiss Prot No：O95985

Mouse Gene Id：21976

Mouse Swiss Prot No：Q9Z321

Immunogen：The antiserum was produced against synthesized peptide derived from human TOP3B. AA range:51-100

Specificity：Topo IIIβ-1 Polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TOP3B;TOP3B1;DNA topoisomerase 3-beta-1;DNA topoisomerase III beta-1

Observed Band(KD)：96kD

Background： This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013],

Function：catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Possesses negatively supercoiled DNA relaxing activity.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,tissue specificity:Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.,

Subcellular Location：condensed chromosome,nucleus,cytosol,

Expression：Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.