Target：Topo IIIα
Fields：Homologous recombination;Fanconi anemia pathway
Gene Name：TOP3A
Protein Name：DNA topoisomerase 3-alpha
Human Gene Id：7156
Human Swiss Prot No：Q13472
Mouse Gene Id：21975
Mouse Swiss Prot No：O70157
Immunogen：Synthesized peptide derived from Topo IIIα . at AA range: 350-430
Specificity：Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：TOP3A;TOP3;DNA topoisomerase 3-alpha;DNA topoisomerase III alpha
Observed Band(KD)：115kD
Background： This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
Function：catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,subunit:Directly interacts with BLM and RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.,tissue specificity:High expression is found in testis, heart, skeletal muscle and pancreas.,
Subcellular Location：Mitochondrion matrix .
Expression：High expression is found in testis, heart, skeletal muscle and pancreas.
