Recommended Dilution Ratio
WB 1:500-2000
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
This antibody detects endogenous levels of hIST1 at Human, Mouse,Rat
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Synthesized peptide derived from human hIST1
Specificity:
This antibody detects endogenous levels of hIST1 at Human, Mouse,Rat
Protein Name:
IST1 homolog (hIST1) (Putative MAPK-activating protein PM28)
Function:
ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation . Is required for efficient abscission during cytokinesis . Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells . During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing . Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 . Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST .
Cellular Localization:
Cytoplasmic vesicle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Midbody . Nucleus envelope . Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the reforming nuclear envelope on chromatin disks during late anaphase. .
