Main Information

Target

ARK-2

Host Species

Rabbit

Reactivity

Human,

Applications

WB, IHC, IF, IP, ELISA

MW

39kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

IHC 1:500-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;

Formulation

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Specificity

Endogenous

Purification

Protein A

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Calculated)

39kD

MW(Observed)

39kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

PT0246R

Isotype

IgG,Kappa

Antigen&Target Information

Specificity:

Endogenous

Gene Name:

AURKB

Protein Name:

Aurora kinase B,Aurora B

Other Name:

AURKB ;
AIK2 ;
AIM1 ;
AIRK2 ;
ARK2 ;
STK1 ;
STK12 ;
STK5 ;
Aurora kinase B ;
Aurora 1 ;
Aurora- and IPL1-like midbody-associated protein 1 ;
AIM-1 ;
Aurora/IPL1-related kinase 2 ;
ARK-2 ;
Aurora-related kinase 2 ;
STK-1 ;
Serine/threonine-protein kinase 12

Database Link:

Organism	Gene ID	SwissProt
Human	9212;	Q96GD4;
Mouse	20877;	O70126;
Rat	114592;	O55099;

Background:

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015],

Function:

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Disease:Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.,Function:May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.,subunit:Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and NDC80. Interacts with EVI5.,tissue specificity:High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.,

Cellular Localization:

Nucleus

Tissue Expression:

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. ; [Isoform 3]: Not expressed in normal liver, high expression in metastatic liver.