Recommended Dilution Ratio
WB 1:500-1000
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Specificity
The antibody detects endogenous Histone H4 (Di Methyl Lys59) protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Synthetic Peptide of Histone H4 (Di Methyl Lys59)
Specificity:
The antibody detects endogenous Histone H4 (Di Methyl Lys59) protein.
Gene Name:
HIST1H4A H4/A H4FA; HIST1H4B H4/I H4FI; HIST1H4C H4/G H4FG; HIST1H4D H4/B H4FB; HIST1H4E H4/J H4FJ; HIST1H4F H4/C H4FC; HIST1H4H H4/H H4FH; HIST1H4I H4/M H4FM; HIST1H4J H4/E H4FE; HIST1H4K H4/D H4FD; HIST1H4L H4/K H4FK; HIST2H4A H4/N H4F2 H4FN HIST2H4; HIST2H4B H4/O H4FO; HIST4H4
Other Name:
H4K59ME2 ;
HIST1H4A ;
H4/A ;
H4FA ;
HIST1H4B ;
H4/I ;
H4FI ;
HIST1H4C ;
H4/G ;
H4FG ;
HIST1H4D ;
H4/B ;
H4FB ;
HIST1H4E ;
H4/J ;
H4FJ ;
HIST1H4F ;
H4/C ;
H4FC ;
HIST1H4H ;
H4/H ;
H4FH ;
HIST1H4I ;
H4/M ;
H4FM ;
HIST1H4J ;
H4/E ;
H4FE ;
HIST1H4K ;
H4/D ;
H4FD ;
HIST1H4L ;
H4/K ;
H4FK ;
HIST2H4A ;
H4/N ;
H4F2 ;
H4FN ;
HIST2H4 ;
HIST2H4B ;
H4/O ;
H4FO ;
HIST4H4 ;
Histone H4
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],
Function:
Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.,PTM:Sumoylated, which is associated with transcriptional repression.,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.,similarity:Belongs to the histone H4 family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,
Cellular Localization:
Nucleus. Chromosome.
Tissue Expression:
B-cell lymphoma,Bone marrow,Brain,Clones donated by HIP,Corpus call
Research Areas:
>>Neutrophil extracellular trap formation ;
>>Alcoholism ;
>>Viral carcinogenesis ;
>>Systemic lupus erythematosus
