Main Information

Target

Nur77

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

MW

77kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

WB 1:500-2000; ELISA(peptide)1:5000-20000

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous pospho levels of human Nuclear Receptor NR4A1 (Phospho-Ser351)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

77kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized pospho peptide derived from human Nuclear Receptor NR4A1 (Phospho-Ser351)

Specificity:

This antibody detects endogenous pospho levels of human Nuclear Receptor NR4A1 (Phospho-Ser351)

Gene Name:

NR4A1 GFRP1 HMR NAK1

Protein Name:

Nuclear Receptor NR4A1 (Phospho-Ser351)

Other Name:

Nuclear receptor subfamily 4 group A member 1 ;
Early response protein NAK1 ;
Nuclear hormone receptor NUR/77 ;
Nur77 ;
Orphan nuclear receptor HMR ;
Orphan nuclear receptor TR3 ;
ST-59 ;
Testicular receptor 3 ;

Database Link:

Organism	Gene ID	SwissProt
Human	3164;	P22736;

Background:

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011],

Function:

Function:Orphan nuclear receptor.,induction:By growth-stimulating agents.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR4 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with GADD45GIP1.,tissue specificity:Fetal muscle and adult liver, brain and thyroid.,

Cellular Localization:

Nucleus . Cytoplasm . Mitochondrion . Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950). .

Tissue Expression:

Fetal muscle and adult liver, brain and thyroid.

Research Areas:

>>MAPK signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>Aldosterone synthesis and secretion ;
>>Cortisol synthesis and secretion ;
>>Cushing syndrome