Main Information

Target

EPHA1

Host Species

Rabbit

Reactivity

Human, Mouse

Applications

IHC, IF, WB

MW

108kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:50-200; WB 1:500-2000; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

This antibody detects endogenous phospho levels of EPHA1 (Phospho-Tyr605) at Human:Y605, Mouse:Y606

Purification

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

108kD

Modification

Phospho

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Synthesized peptide derived from human EPHA1 (Phospho-Tyr605)

Specificity:

This antibody detects endogenous phospho levels of EPHA1 (Phospho-Tyr605) at Human:Y605, Mouse:Y606

Gene Name:

EPHA1 EPH EPHT EPHT1

Protein Name:

EPHA1 (Phospho-Tyr605)

Other Name:

Ephrin type-A receptor 1 ;
hEpha1 ;
EPH tyrosine kinase ;
EPH tyrosine kinase 1 ;
Erythropoietin-producing hepatoma receptor ;
Tyrosine-protein kinase receptor EPH ;

Database Link:

Organism	Gene ID	SwissProt
Human	2041;	P21709;

Background:

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008],

Function:

Catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,Function:Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,tissue specificity:Overexpressed in several carcinomas.,

Cellular Localization:

Cell membrane ; Single-pass type I membrane protein .

Tissue Expression:

Overexpressed in several carcinomas.

Research Areas:

>>Axon guidance