Main Information

Target

CaMKIIβ/γ/δ

Host Species

Mouse

Reactivity

Human, Rat, Mouse

Applications

IHC, IF

MW

50kD (Observed)

Conjugate/Modification

Phospho

Detailed Information

Recommended Dilution Ratio

IHC 1:100-200; IF 1:50-200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

CaMKIIβ/γ/δ (Phospho Thr287) protein detects endogenous levels of CaMKIIβ/γ/δ (Phospho Thr287)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

50kD

Modification

Phospho

Clonality

Monoclonal

Clone Number

4H2

Antigen&Target Information

Immunogen:

Synthetic Peptide of CaMKIIβ/γ/δ (Phospho Thr287)

Specificity:

CaMKIIβ/γ/δ (Phospho Thr287) protein detects endogenous levels of CaMKIIβ/γ/δ (Phospho Thr287)

Other Name:

CAMK2B ;
CAM2 ;
CAMK2 ;
CAMKB ;
Calcium/calmodulin-dependent protein kinase type II subunit beta ;
CaM kinase II subunit beta ;
CaMK-II subunit beta ;
CAMK2G ;
CAMK ;
CAMK-II ;
CAMKG ;
Calcium/calmodulin-dependent protein kinase type II subunit gamma ;

Database Link:

Organism	Gene ID	SwissProt
Human	816;817;818;	Q13554;Q13555;Q13557;

Background:

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],

Function:

Alternative products:The variable region of the CAMK2B protein is encoded by at least 7 exons (V1 to V7). Alternative splicing within this region gives rise to CAMK2B isoforms,Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.,Function:CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.,similarity:Contains 1 protein kinase domain.,subunit:CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ.,tissue specificity:Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.,

Cellular Localization:

Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Sarcoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.

Tissue Expression:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

Research Areas:

>>ErbB signaling pathway ;
>>Calcium signaling pathway ;
>>cAMP signaling pathway ;
>>HIF-1 signaling pathway ;
>>Oocyte meiosis ;
>>Necroptosis ;
>>Adrenergic signaling in cardiomyocytes ;
>>Wnt signaling pathway ;
>>Axon guidance ;
>>Circadian entrainment ;
>>Long-term potentiation ;
>>Neurotrophin signaling pathway ;
>>Cholinergic synapse ;
>>Dopaminergic synapse ;
>>Olfactory transduction ;
>>Inflammatory mediator regulation of TRP channels ;
>>Insulin secretion ;
>>GnRH signaling pathway ;
>>Melanogenesis ;
>>Oxytocin signaling pathway ;
>>Glucagon signaling pathway ;
>>Aldosterone synthesis and secretion ;
>>Cushing syndrome ;
>>Gastric acid secretion ;
>>Parkinson disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Amphetamine addiction ;
>>Tuberculosis ;
>>Pathways in cancer ;
>>Proteoglycans in cancer ;
>>Glioma ;
>>Diabetic cardiomyopathy ;
>>Lipid and atherosclerosis