Main Information

Target

A-FABP

Host Species

Mouse

Reactivity

Human

Applications

WB, ELISA

MW

15kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

A-FABP Monoclonal Antibody detects endogenous levels of A-FABP protein.

Purification

Affinity purification

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Calculated)

15kD

Modification

Unmodified

Clonality

Monoclonal

Antigen&Target Information

Immunogen:

Purified recombinant fragment of A-FABP (aa61-121) expressed in E. Coli.

Specificity:

A-FABP Monoclonal Antibody detects endogenous levels of A-FABP protein.

Gene Name:

FABP4

Protein Name:

Fatty acid-binding protein, adipocyte

Other Name:

FABP4 ;
Fatty acid-binding protein ;
adipocyte ;
Adipocyte lipid-binding protein ;
ALBP ;
Adipocyte-type fatty acid-binding protein ;
A-FABP ;
AFABP ;
Fatty acid-binding protein 4

Database Link:

Organism	Gene ID	SwissProt
Human	2167;	P15090;
Mouse		P04117;

Background:

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008],

Function:

Domain:Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.,Function:Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.,similarity:Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.,subcellular location:Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.,subunit:Homodimer. Interacts with PPARG (By similarity). Monomer.,

Cellular Localization:

Cytoplasm . Nucleus . Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. .

Tissue Expression:

Urinary bladder,

Research Areas:

>>PPAR signaling pathway ;
>>Regulation of lipolysis in adipocytes