Main Information

Target

AATM

Host Species

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IF, ELISA

MW

48kD (Calculated)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

AATM Monoclonal Antibody detects endogenous levels of AATM protein.

Purification

Affinity purification

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Calculated)

48kD

Modification

Unmodified

Clonality

Monoclonal

Antigen&Target Information

Immunogen:

Purified recombinant fragment of human AATM expressed in E. Coli.

Specificity:

AATM Monoclonal Antibody detects endogenous levels of AATM protein.

Gene Name:

GOT2

Protein Name:

Aspartate aminotransferase, mitochondrial

Other Name:

GOT2 ;
Aspartate aminotransferase ;
mitochondrial ;
mAspAT ;
Fatty acid-binding protein ;
FABP-1 ;
Glutamate oxaloacetate transaminase 2 ;
Kynurenine aminotransferase 4 ;
Kynurenine aminotransferase IV ;
Kynurenine--oxoglutarate transaminase 4 ;
Kynu

Database Link:

Organism	Gene ID	SwissProt
Human	2806;	P00505;
Mouse	14719;	P05202;
Rat	25721;	P00507;

Background:

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013],

Function:

Catalytic activity:L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.,cofactor:Pyridoxal phosphate.,Function:Facilitates cellular uptake of long-chain free fatty acids.,miscellaneous:In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer.,

Cellular Localization:

Mitochondrion matrix . Cell membrane . Exposure to alcohol promotes translocation to the cell membrane. .

Tissue Expression:

Epithelium,Gastric mucosa,Muscle,

Research Areas:

>>Arginine biosynthesis ;
>>Alanine, aspartate and glutamate metabolism ;
>>Cysteine and methionine metabolism ;
>>Arginine and proline metabolism ;
>>Tyrosine metabolism ;
>>Phenylalanine metabolism ;
>>Phenylalanine, tyrosine and tryptophan biosynthesis ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>2-Oxocarboxylic acid metabolism ;
>>Biosynthesis of amino acids ;
>>Fat digestion and absorption