Main Information

Target

Tubulin β

Host Species

Mouse

Reactivity

Human, Rat, Mouse, Mk, Dg, Ch, Hamster, Rabbit, sheep, Insect, Yeast

Applications

WB, IF, IHC

MW

55kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-1:3000; IHC 1:50-1:200; IF 1:200

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

Theβ-tubulin antibody can detects endogenousβ-tubulin protein.

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

55kD

Modification

Unmodified

Clonality

Monoclonal

Clone Number

5G3

Antigen&Target Information

Immunogen:

Recombinant Protein of β-tubulin (HRP Conjugated)

Specificity:

Theβ-tubulin antibody can detects endogenousβ-tubulin protein.

Gene Name:

TUBB

Protein Name:

Tubulin beta chain (Tubulin beta-5 chain)

Other Name:

Tubulin beta chain ;
Tubulin beta-5 chain ;

Database Link:

Organism	Gene ID	SwissProt
Human	203068;	P07437;
Mouse		P99024;
Rat		P69897;

Background:

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014],

Function:

Domain:The highly acidic C-terminal region may bind cations such as calcium.,Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,online information:Tubulin entry,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,tissue specificity:Ubiquitously expressed with highest levels in spleen, thymus and immature brain.,

Cellular Localization:

Cytoplasm, cytoskeleton .

Tissue Expression:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Research Areas:

>>Phagosome ;
>>Gap junction ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection