Main Information

Target

His-tag

Host Species

Rabbit

Reactivity

Species independent

Applications

WB, ELISA

MW

15kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:1000-1:3000; ELISA 1:20000; Not yet tested in other applications.

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity

His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

Concentration

1 mg/ml

MW(Observed)

15kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

6X His synthetic peptide conjugated to KLH.

Specificity:

His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Gene Name:

His-Tag

Protein Name:

His Tag

Other Name:

6 His epitope tag ;
Hexa His tag ;
HHHHHH epitope tag ;
HHHHHH tag ;
His tag

Background:

The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.