Recommended Dilution Ratio
IHC 1:50-200; WB 1:500-2000; IF 1:50-200
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Specificity
This antibody detects endogenous levels of human PARP-1
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Immunogen:
Synthesized peptide derived from human PARP-1. AA range: 410-460
Specificity:
This antibody detects endogenous levels of human PARP-1
Gene Name:
PARP1 ADPRT PPOL
Other Name:
Poly [ADP-ribose] polymerase 1 ;
PARP-1 ;
ADP-ribosyltransferase diphtheria toxin-like 1 ;
ARTD1 ;
NAD ;
+ ;
ADP-ribosyltransferase 1 ;
ADPRT 1 ;
Poly[ADP-ribose] synthase 1 ;
Database Link:
| Organism |
Gene ID |
SwissProt |
| Human |
142; |
P09874; |
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
Function:
Catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,Function:Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.,miscellaneous:The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Poly-ADP-ribosylated by PARP2.,similarity:Contains 1 BRCT domain.,similarity:Contains 1 PARP alpha-helical domain.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 2 PARP-type zinc fingers.,subunit:Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423.,
Cellular Localization:
Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. .
Tissue Expression:
Brain,Colon carcinoma,Fibroblast,Lung,Ovarian carcinoma,Skin,
Research Areas:
>>Base excision repair ;
>>NF-kappa B signaling pathway ;
>>Apoptosis ;
>>Necroptosis ;
>>Diabetic cardiomyopathy
