Main Information

Target

SLUG

Host Species

Rabbit

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

MW

30kD (Observed)

Conjugate/Modification

Unmodified

Detailed Information

Recommended Dilution Ratio

WB 1:500-1000; IHC 1:200-500; IF 1:50-200

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Specificity

The antibody detects endogenous SLUG protein.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-15°C to -25°C/1 year(Do not lower than -25°C)

MW(Observed)

30kD

Modification

Unmodified

Clonality

Polyclonal

Isotype

IgG

Antigen&Target Information

Immunogen:

Recombinant Protein of SLUG

Specificity:

The antibody detects endogenous SLUG protein.

Gene Name:

SNAI2

Protein Name:

Zinc finger protein SNAI2

Other Name:

SNAI2 ;
SLUG ;
SLUGH ;
Zinc finger protein SNAI2 ;
Neural crest transcription factor Slug ;
Protein snail homolog 2

Database Link:

Organism	Gene ID	SwissProt
Human	6591;	O43623;
Mouse		P97469;
Rat		O08954;

Background:

snail family transcriptional repressor 2(SNAI2) Homo sapiens This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008],

Function:

Disease:Defects in SNAI2 are a cause of neural tube defects (NTD).,Disease:Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.,Function:Transcriptional repressor. Involved in the generation and migration of neural crest cells.,similarity:Belongs to the snail C2H2-type zinc-finger protein family.,similarity:Contains 5 C2H2-type zinc fingers.,tissue specificity:Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.,

Cellular Localization:

Nucleus . Cytoplasm. Observed in discrete foci in interphase nuclei. These nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting SNAI2 is associated with active transcription or active splicing regions.

Tissue Expression:

Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level).

Research Areas:

>>Hippo signaling pathway ;
>>Adherens junction