产品简介:
pMXB10 is a control plasmid for the IMPACT Kit. This plasmid carries the E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa), fused in-frame to the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1. pMXB10 can be used to test plasmid transformation, cell culture, induction and purification procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours), 100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector is 7813 bp in length.
产品参数:
质粒类型:大肠杆菌表达载体
出品公司:NEB
克隆方法:多克隆位点,限制性内切酶启动子:T7
载体大小:7817 bp
检测标签:无
载体抗性:Ampicillin (氨苄青霉素)
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗等其他用途。
2、使用甘油菌可不完全融解,在菌液表面蘸取少量涂布固体琼脂平板即可。完全融解后使用,菌体会逐渐沉淀至管底,请混匀后使用。随着冻融次数的增加,菌株的活力会逐渐下降。