载体介绍：

ADDITIONAL MATERIALS REQUIRED

T4 DNA ligase

Taq DNA polymerase

Taq DNA polymerase buffer

TE buffer

CIAP

Falcon 2059 polypropylene tubes (15-ml)

Water baths (37°C and 42°C)

 

XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)].

The XL1-Blue cells supplied with the pCMV-Tag vectors are not competent cells. Refer to Hanahan (1983) for a protocol for producing competent cells.

产品参数：

质粒类型：哺乳动物表达载体

高拷贝/低拷贝:高拷贝

克隆方法:CMV

启动子：CMV

载体大小：4327 bp 

5' 测序引物及序列：T3:AATTAACCCTCACTAAAGGG

3' 测序引物及序列：T7:GTAATACGACTCACTATAGGGC

载体标签：Myc

载体抗性：Kanamycin (卡那霉素)

筛选标记：Neo(新霉素)