载体介绍:
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
产品参数:
质粒类型:哺乳动物表达载体
启动子:CMV
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:10186 bp
5' 测序引物:CMV-F:5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物:EBV-R:5'-GTGGTTTGTCCAAACTCATC-3'
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Hygromycin (潮霉素)