载体介绍:
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
质粒类型:哺乳动物表达载体
启动子:UbC
克隆方法:多克隆位点,限制性内切酶
载体大小:5467 bp
5' 测序引物及序列:T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
载体标签:His Tag (6x), V5 Epitope Tag
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Blasticidin