载体介绍:

pcDNA™4/HisMax is specifically designed to maximize protein expression in a variety of mammalian cells. The vector contains the QBI SP163 translational enhancer to increase expression levels two- to five-fold above those seen with the promoter alone. In addition to SP163-enhanced expression, pcDNA™4/HisMax includes a cleavable N-terminal Xpress™ tag for rapid detection of recombinant protein with an Anti-Xpress™ Antibody. pcDNA™4/HisMax is available TOPO® Cloning-ready for five-minute cloning of Taqamplified PCR products.

产品参数:

质粒类型：哺乳细胞表达载体

高拷贝/低拷贝：高拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：5130bp 

5' 测序引物及序列：T7-F:5'd[TAATACGACTCACTATAGGG]3'

载体标签：6X His, Xpress

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Zeocin (博莱霉素)