产品简介：

The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

 

产品参数：
质粒类型：昆虫细胞表达系统

出品公司：Novagen

启动子：IE1,p10

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：6802 bp 

载体标签：C-His,N-Strep 

载体抗性：Ampicillin (氨苄青霉素)

备注：带有hr5增强子