描述

Proteomic approaches require simple and efficient protein purification methodologies that are amenable to high throughput. Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/
streptavidin for biotinylated templates. Here, an approach for the single-step purification of protein complex(es) based on specific in vivo biotinylation. Expressed the bacterial BirA biotin ligase in mammalian cells and demonstrated very efficient biotinylation of the Biotin-tag. Biotinylation of the tagged protein altered neither the protein interactions or DNA binding properties in vivo nor its subnuclear distribution.

详细说明

出品公司：Transvector

载体类型：蛋白检测系统

骨架载体：pcDNA3.1-Myc-His A  

启动子：CMV

关键功能：BirA表达

原核抗性：Amp

真核抗性：Neomycin

真核检测：RFP