载体介绍：

pLVX-EF1α-IRES-Puro is an HIV-1-based, lentiviral expression vector designed to simultaneously and constitutively express a protein of interest and puromycin resistance (Puror) from a bicistronic transcript in mammalian cells. Expression of your protein of interest and Puror from a bicistronic transcript allows puromycin resistance to be used as an indicator of transduction efficiency and a marker for selection.

产品参数：

质粒类型：慢病毒载体

高拷贝/低拷贝：高拷贝

启动子：EF1α/ EF1a

克隆方法：多克隆位点，限制性内切酶

载体大小：8825 bp

5' 测序引物及序列：EF-1a Forward:TCAAGCCTCAGACAGTGGTTC

3' 测序引物及序列：IRES-R:CCTCACATTGCCAAAAGACG

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：嘌呤霉素(Puromycin)

备注：含有IRES序列和EF1a启动子，慢病毒表达载体