出品公司：HCY

详情描述：

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

新型NgAgo基因编辑系统质粒载体，载体骨架pEGFP-N1, NgAgo基因N端带有SV40 NLS核内定位信号肽，CMV启动子，Kan抗性-G418真核细胞筛选标记。BioVector NTCC质粒载体菌种细胞基因保藏中心提供

Vector backbone

pEGFP-N1

Backbone size w/o insert (bp)4000

Total vector size (bp)6679

Modifications to backbone

pEGFP-N1 without the eGFP

Vector type

Mammalian Expression

Selectable markers

Neomycin (select with G418)

Bacterial Resistance(s)

Kanamycin

Growth Temperature

37°C

Growth Strain(s)

DH5alpha

Gene/Insert name

NgAgo

Alt name

Argonaute

Species

Natronobacterium gregoryi

Insert Size (bp)

2712

GenBank ID

AFZ73749.1

PromoterCMV

Tag / Fusion Protein

a nuclear localization signal (NLS) (N terminal on insert)

Cloning methodRestriction Enzyme

5′ cloning siteHindIII (not destroyed)

3′ cloning siteBamHI (not destroyed)

5′ sequencing primerCMV-F

3′ sequencing primerSV40pA-R