BACKBONE

Vector backbone

pCR8/GW/TOPO

Backbone size w/o insert (bp)2799

Total vector size (bp)7442

Vector type

CRISPR ; Gateway Donor

 

GENE/INSERT

Gene/Insert namedCas9(D10A;H840A) fusion with VP160 activation domain
Alt namedCas9VP160
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)4643
MutationD10A;H840A nuclease-deficient
Tags / Fusion Proteins
HA Tag (N terminal on insert)
VP160 (C terminal on insert)

 

GROWTH IN BACTERIA

Bacterial Resistance(s)

Spectinomycin

Growth Temperature

37°C

Growth Strain(s)

DH5alpha

Copy number

Unknown

 

CLONING INFORMATION

Cloning method：Restriction Enzyme

5′ cloning site：EcoRI (not destroyed)

3′ cloning site：EcoRI (not destroyed)

5′ sequencing primer：M13F

3′ sequencing primer：M13R