Basic product information:

 

质粒简介：

 pECFP-C1 encodes an enhanced cyan fluorescent variant of the Aequorea victoria green fluorescent protein gene (GFP). The ECFP gene contains six amino acid substitutions. The Tyr-66 to Trp substitution gives ECFP fluorescence excitation (major peak at 433 nm and a minor peak at 453 nm) and emission (major peak at 475 nm and a minor peak at 501 nm) similar to other cyan emission variants (1–3). The other five substitutions (Phe-64 to Leu; Ser-65 to Thr; Asn-146 to Ile; Met-153 to Thr; and Val-163 to Ala) enhance the brightness and solubility of the protein, primarily due to improved protein-folding properties and efficiency of chromophore formation (2, 4, 5).

 

                                                

 

 

产品参数：

出品公司：Clontech

启动子: CMV

复制子: pUC ori,f1 ori

终止子: SV40 poly(A) signal

质粒分类: 哺乳细胞，荧光蛋白报告载体

质粒大小: 4731bp

原核抗性: Kan

筛选标记: Neo

克隆菌株: DH5α

培养条件: 37℃，有氧 LB

表达宿主: 哺乳细胞

诱导方式: 无须诱导，瞬时表达

5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG

3'测序引物: Sv40-polyA-R：GAAATTTGTGATGCTATTGC

Other technical information

For more information, please download and refer to the product manual。