产品简介：

The Flp-In System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location.The Flp-In System involves introduction of a Flp

Recombination Target (FRT) site into the genome of the mammalian cell line of choice.An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site. The second major component of the Fp-In System is the pcDNA5/FRT expression vector into which the gene of interest will be cloned. Expression of the gene of interest is controlled by the human CMV promoter. The vector also contains the hygromycin resistance gene with a FRT site embedded in the 5 coding region. The hvaromycin resistance gene lacks a promoter and the ATG initiation codon.

pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System.

This vector features:

1. CMV promoter for high level expression in mammalian cells;

2. ten unique restriction site for easy cloning;

3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line;

4. Hygromycin resistance gene for selection of stable cell lines.

 

产品参数：

质粒类型：FLP/FRT位点特异性重组系统

出品公司：Invitrogen

启动子：CMV

载体大小：5070 bp 

载体抗性：Ampicillin

筛选标记：Hygromycin