产品简介：

pOG44 is a 5.8 kb Flp recombinase expression vector designed for use with the Flp-In. System (Catalog nos. K6010-01 and K6010-02) available from Invitrogen. When cotransfected with the pcDNA5/FRT plasmid into a Flp-In. mammalian host cell line, the Flp recombinase expressed from pOG44 mediates integration of the pcDNA5/FRT vector containing the gene of interest into the genome via Flp Recombination Target (FRT) sites. 

The vector contains the following elements:

·The human cytomegalovirus (CMV) immediate-early enhancer/promoter for highlevel constitutive expression of the Flp recombinase in a wide range of mammalian cells (Andersson et al., 1989; Boshart et al., 1985; Nelson et al., 1987)

·Synthetic intron to enhance expression of the FLP gene (Huang and Gorman, 1990; O’Gorman et al., 1991)

·FLP gene encoding the Flp recombinase (Buchholz et al., 1996) to mediate integration of the pcDNA5/FRT expression plasmid into the genome

 

产品参数：

质粒类型：哺乳细胞表达载体

出品公司：Invitrogen

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：5785bp 

载体抗性：Ampicillin (氨苄青霉素)