产品简介：

The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System. The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains. The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted. You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit.

 

产品参数：

载体别名：pPink(alpha)-HC

出品公司：Invitrogen

质粒类型：酵母表达载体

表达水平：低拷贝

启动子：AOX1 promoter

克隆方法：多克隆位点，限制性内切酶

载体大小：7898 bp

5' 测序引物：5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´  alpha-Factor-F:5´-TACTATTGCCAGCATTGCTGC-3´

3' 测序引物：3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´

载体标签：N-alpha 分泌信号肽

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：ADE2

备注：拥有alpha因子，分泌表达蛋白