Basic product information:

 

质粒简介：

pDsRed-Monomer-N1 is a mammalian expression vector that encodes DsRed-Monomer (DsRed.M1), a monomeric mutant derived from the tetrameric Discosoma sp. red fuorescent protein DsRed. DsRed-Monomer contains forty-fve amino acid substitutions (listed on page 2). When DsRed-Monomer is expressed in mammalian cell cultures, red fuorescent cells can be detected by either fuorescence microscopy or fow cytometry 12–16 hr after transfection (DsRed-Monomer excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence is human codon-optimized for high expression in mammalian cells.

 

 

                                     

产品参数：

出品公司：Clontech

启动子: CMV

复制子: pUC ori,f1 ori

终止子: SV40 poly(A) signal

质粒分类: 哺乳细胞，荧光蛋白报告载体

质粒大小: 4691bp

原核抗性: Kan

筛选标记: Neo

克隆菌株: DH5α

培养条件: 37℃，有氧 LB

表达宿主: 哺乳细胞

诱导方式: 无须诱导，瞬时表达

5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG

 

3'测序引物: 根据序列设计引物

Other technical information

For more information, please download and refer to the product manual。