Target:LC3B
Fields:Mitophagy - animal;Autophagy - animal;Ferroptosis;Apelin signaling pathway;NOD-like receptor signaling pathway;Amyotrophic lateral sclerosis;Pathways of neurodegeneration - multiple diseases;Shigellosis;Kaposi sarcoma-associated herpesvirus infection
Gene Name:MAP1LC3B
Protein Name:MAP1LC3B
Human Gene Id:81631
Human Swiss Prot No:Q9GZQ8
Mouse Swiss Prot No:Q9CQV6
Immunogen:Recombinant Protein of LC3B of MAP1LC3B
Specificity:LC3B protein detects endogenous levels of MAP1LC3B
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Monoclonal, Mouse
Dilution:WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000. IF 1:50-200
Purification:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:MAP1LC3B
Observed Band(KD):14,16kD
Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008],
Function:caution:PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.,function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.,
Subcellular Location:Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Mitochondrion membrane ; Lipid-anchor . Cytoplasm, cytoskeleton . Cytoplasmic vesicle . LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme. .
Expression:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.