Target：NOS3
Fields：Arginine biosynthesis;Arginine and proline metabolism;Metabolic pathways;Calcium signaling pathway;cGMP-PKG signaling pathway;HIF-1 signaling pathway;Sphingolipid signaling pathway;PI3K-Akt signaling pathway;VEGF signaling pathway;Apelin signaling pathway;Platelet activation;Estrogen signaling pathway;Oxytocin signaling pathway;Relaxin signaling pathway;Insulin resistance;AGE-RAGE signaling pathway in diabetic complications;Diabetic cardiomyopathy;Lipid and atherosclerosis;Fluid shear stress and atherosclerosis
Gene Name：NOS3
Protein Name：Nitric oxide synthase, endothelial
Human Gene Id：4846
Human Swiss Prot No：P29474
Mouse Gene Id：18127
Mouse Swiss Prot No：P70313
Rat Gene Id：24600
Rat Swiss Prot No：Q62600
Immunogen：Recombinant Protein of eNOS
Specificity：The antibody detects endogenous eNOS protein.
Formulation：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source：Monoclonal, Mouse
Dilution：WB 1:500-2000
Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：NOS3;Nitric oxide synthase, endothelial;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;NOS type III;NOSIII
Observed Band(KD)：130-140kD
Background： Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Function：catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like
Subcellular Location：Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Expression：Platelets, placenta, liver and kidney.
