Target：Insulin
Fields：MAPK signaling pathway;Ras signaling pathway;Rap1 signaling pathway;cGMP-PKG signaling pathway;HIF-1 signaling pathway;FoxO signaling pathway;Phospholipase D signaling pathway;Oocyte meiosis;Autophagy - animal;mTOR signaling pathway;PI3K-Akt signaling pathway;AMPK signaling pathway;Longevity regulating pathway;Longevity regulating pathway - multiple species;Regulation of actin cytoskeleton;Insulin signaling pathway;Insulin secretion;Ovarian steroidogenesis;Progesterone-mediated oocyte maturation;Prolactin signaling pathway;Regulation of lipolysis in adipocytes;Type II diabetes mellitus;Insulin resistance;Non-alcoholic fatty liver disease;Type I diabetes mellitus;Maturity onset diabetes of the young;Aldosterone-regulated sodium reabsorption;Alzheimer disease;Prostate cancer;Diabetic cardiomyopathy
Gene Name：INS
Protein Name：Insulin [Cleaved into: Insulin B chain; Insulin A chain]
Human Gene Id：3630
Human Swiss Prot No：P01308
Immunogen：Synthesized peptide derived from human Insulin AA range: 25-110
Specificity：The antibody can specifically recognize human Insulin protein.
Formulation：PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source：Mouse, Monoclonal/IgG2b, kappa
Dilution：IHC 1:200-400. ELISA 1:500-5000
Purification：The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Molecular Weight(Da)：12kD
Observed Band(KD)：9kD
Background： After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],
Function：disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insu
Subcellular Location：Cytoplasmic
Expression： Blood,Liver,Muscle,Pancreas,
