Target：Ku70/XRCC6
Fields：Non-homologous end-joining
Gene Name：XRCC6
Protein Name：X-ray repair cross-complementing protein 6
Human Gene Id：2547
Human Swiss Prot No：P12956
Mouse Gene Id：14375
Mouse Swiss Prot No：P23475
Immunogen：The antiserum was produced against synthesized Acetyl-peptide derived from human Ku70 around the Acetylation site of Lys331. AA range:291-340
Specificity：Acetyl-Ku-70 (K331) Polyclonal Antibody detects endogenous levels of Ku-70 protein only when acetylated at K331.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500-2000;IHC 1:50-300
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：XRCC6;G22P1;X-ray repair cross-complementing protein 6;5'-deoxyribose-5-phosphate lyase Ku70;5'-dRP lyase Ku70;70 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 1;ATP-dependent DNA helicase II 70 kDa subunit;CTC box-binding factor 75 kDa subunit;CTC75;CTCBF;DNA repair protein XRCC6;Lupus Ku autoantigen protein p70;Ku70;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 6
Observed Band(KD)：70kD
Background： The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008],
Function：developmental stage:Expression does not increase during promyelocyte differentiation.,disease:Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86. Existence of a major autoantigenic epitope or epitopes on the C-terminal 190 amino acids of p70 containing the leucine repeat. The majority of autoantibodies to p70 in most sera from patients with SLE seem to be reactive with this region.,function:Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86
Subcellular Location：Nucleus . Chromosome .
Expression： Brain,Cervix carcinoma,Epithelium,Heart,Hepatocyte,Kidney,Liver,Lun
