Target：NFkB p65
Fields：Antifolate resistance;MAPK signaling pathway;Ras signaling pathway;cAMP signaling pathway;Chemokine signaling pathway;NF-kappa B signaling pathway;HIF-1 signaling pathway;Sphingolipid signaling pathway;Mitophagy - animal;PI3K-Akt signaling pathway;Apoptosis;Longevity regulating pathway;Cellular senescence;Osteoclast differentiation;Neutrophil extracellular trap formation;Toll-like receptor signaling pathway;NOD-like receptor signaling pathway;RIG-I-like receptor signaling pathway;Cytosolic DNA-sensing pathway;C-type lectin receptor signaling pathway;IL-17 signaling pathway;Th1 and Th2 cell differentiation;Th17 cell differentiation;T cell receptor signaling pathway;B cell receptor signaling pathway;TNF signaling pathway;Neurotrophin signaling pathway;Prolactin signaling pathway;Adipocytokine signaling pathway;Relaxin signaling pathway;Insulin resistance;Non-alcoholic fatty liver disease;AGE-RAGE signaling pathway in diabe
Gene Name：RELA
Protein Name：Transcription factor p65
Human Gene Id：5970
Human Swiss Prot No：Q04206
Mouse Gene Id：19697
Mouse Swiss Prot No：Q04207
Immunogen：Synthesized acetyl-peptide derived from the Internal region of human NFκB-p65 around the acetylation site of K221.
Specificity：Acetyl-NFκB-p65 (K221) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylation at K221.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Observed Band(KD)：65kD
Background： NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Function：function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by in
Subcellular Location：Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .
Expression： Bone,Colon,Pancreas,Placenta,
