Target：PKD1/2/3

Fields：Rap1 signaling pathway;Aldosterone synthesis and secretion;Chemical carcinogenesis - reactive oxygen species

Gene Name：KPCD1/KPCD2/KPCD3

Protein Name：Serine/threonine-protein kinase D1/2/3

Human Gene Id：5587/25865/23683

Human Swiss Prot No：Q15139/Q9BZL6/O94806

Mouse Gene Id：18760/101540/75292

Rat Gene Id：85421/292658

Rat Swiss Prot No：Q9WTQ1/Q5XIS9

Immunogen：The antiserum was produced against synthesized peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742. AA range:706-755

Specificity：Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody detects endogenous levels of PKD1/2/3 protein only when phosphorylated at S738/S742.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：PRKD1;PKD;PKD1;PRKCM;Serine/threonine-protein kinase D1;Protein kinase C mu type;Protein kinase D;nPKC-D1;nPKC-mu;PRKD2;PKD2;HSPC187;Serine/threonine-protein kinase D2;nPKC-D2;PRKD3;EPK2;PRKCN;Serine/threonine-protein kinas

Observed Band(KD)：101kD

Background： PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],

Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress.,PTM:Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src.,

Subcellular Location：Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation.

Expression： Placenta,Testis,