Target:WEE1
Fields:Cell cycle;Human immunodeficiency virus 1 infection
Gene Name:WEE1
Protein Name:Wee1-like protein kinase
Human Gene Id:7465
Human Swiss Prot No:P30291
Mouse Gene Id:22390
Mouse Swiss Prot No:P47810
Rat Gene Id:308937
Rat Swiss Prot No:Q63802
Immunogen:The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53. AA range:19-68
Specificity:Phospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:WEE1;Wee1-like protein kinase;WEE1hu;Wee1A kinase
Molecular Weight(Da):72kD
Background:WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],
Function:catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase
Subcellular Location:Nucleus.
Expression: Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,