Target：Tubulin α

Fields：Phagosome;Apoptosis;Tight junction;Gap junction;Alzheimer disease;Parkinson disease;Amyotrophic lateral sclerosis;Huntington disease;Prion disease;Pathways of neurodegeneration - multiple diseases;Pathogenic Escherichia coli infection;Salmonella infection

Gene Name：TUBA1A

Protein Name：Tubulin alpha-1A chain

Human Gene Id：7846/10376/84790/113457/7278/112714/7277

Human Swiss Prot No：Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366

Mouse Gene Id：22142/22143/22146

Rat Gene Id：64158/500929/100909441

Rat Swiss Prot No：P68370/Q6P9V9/Q6AYZ1

Immunogen：The antiserum was produced against synthesized peptide derived from human TUBA1/3/4 around the phosphorylation site of Tyr272. AA range:238-287

Specificity：Phospho-Tubulin α1/3/4 (Y272) Polyclonal Antibody detects endogenous levels of Tubulin α1/3/4 protein only when phosphorylated at Y272.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：TUBA1A;TUBA3;Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1B;Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain;TUBA1C;TUBA6;Tubulin alpha-1C c

Observed Band(KD)：50-55kD

Background： Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.

Function：disease:Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem.,function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), resp

Subcellular Location：Cytoplasm, cytoskeleton.

Expression：Expressed at a high level in fetal brain.