Target：4E-BP1

Gene Name：EIF4EBP1

Protein Name：Eukaryotic translation initiation factor 4E-binding protein 1

Human Swiss Prot No：Q13541

Mouse Gene Id：13685

Rat Gene Id：116636

Rat Swiss Prot No：Q62622

Immunogen：The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. AA range:35-84

Specificity：Phospho-4E-BP1 (T69) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T69.

Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source：Polyclonal, Rabbit,IgG

Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration：1 mg/ml

Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)

Other Name：EIF4EBP1;Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I

Observed Band(KD)：12kD

Background：EIF4EBP1 encodes one member of a family of translation repressor proteins. The eukaryotic translation initiation factor 4E binding protein 1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.