Target:HSP90B
Fields:Protein processing in endoplasmic reticulum;PI3K-Akt signaling pathway;Necroptosis;Antigen processing and presentation;NOD-like receptor signaling pathway;IL-17 signaling pathway;Th17 cell differentiation;Progesterone-mediated oocyte maturation;Estrogen signaling pathway;Salmonella infection;Pathways in cancer;Chemical carcinogenesis - receptor activation;Prostate cancer;Lipid and atherosclerosis;Fluid shear stress and atherosclerosis
Gene Name:HSP90AB1
Protein Name:Heat shock protein HSP 90-beta
Human Gene Id:3326
Human Swiss Prot No:P08238
Mouse Gene Id:15516
Mouse Swiss Prot No:P11499
Rat Gene Id:301252
Rat Swiss Prot No:P34058
Immunogen:The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275
Specificity:Phospho-HSP90β (S254) Polyclonal Antibody detects endogenous levels of HSP90β protein only when phosphorylated at S254.
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name:HSP90AB1;HSP90B;HSPC2;HSPCB;Heat shock protein HSP 90-beta;HSP 90;Heat shock 84 kDa;HSP 84;HSP84
Observed Band(KD):90kD
Background: This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],
Function:function:Molecular chaperone. Has ATPase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with TP53/p53 (By similarity). Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer.,
Subcellular Location:Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). .
Expression: Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon