Target：SPAK
Gene Name：STK39
Protein Name：STE20/SPS1-related proline-alanine-rich protein kinase
Human Gene Id：27347
Human Swiss Prot No：Q9UEW8
Mouse Gene Id：53416
Mouse Swiss Prot No：Q9Z1W9
Rat Gene Id：54348
Rat Swiss Prot No：O88506
Immunogen：The antiserum was produced against synthesized peptide derived from human STK39 around the phosphorylation site of Ser325. AA range:291-340
Specificity：Phospho-SPAK (S325) Polyclonal Antibody detects endogenous levels of SPAK protein only when phosphorylated at S325.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：STK39;SPAK;STE20/SPS1-related proline-alanine-rich protein kinase;Ste-20-related kinase;DCHT;Serine/threonine-protein kinase 39
Molecular Weight(Da)：60kD
Background： This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008],
Function：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:PAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.,function:May act as a mediator of stress-activated signals.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Nucleus when caspase-cleaved.,tissue specificity:Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.,
Subcellular Location：Cytoplasm . Nucleus . Nucleus when caspase-cleaved. .
Expression：Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
