Target：Sp1
Fields：Endocrine resistance;Mitophagy - animal;TGF-beta signaling pathway;Estrogen signaling pathway;Cortisol synthesis and secretion;Parathyroid hormone synthesis, secretion and action;Cushing syndrome;Huntington disease;Spinocerebellar ataxia;Human cytomegalovirus infection;Pathways in cancer;Transcriptional misregulation in cancer;Breast cancer;Choline metabolism in cancer;Diabetic cardiomyopathy
Gene Name：SP1
Protein Name：Transcription factor Sp1
Human Gene Id：6667
Human Swiss Prot No：P08047
Mouse Gene Id：20683
Mouse Swiss Prot No：O89090
Rat Gene Id：24790
Rat Swiss Prot No：Q01714
Immunogen：The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr739. AA range:706-755
Specificity：Phospho-Sp1 (T739) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T739.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Polyclonal, Rabbit,IgG
Dilution：WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-15°C to -25°C/1 year(Do not lower than -25°C)
Other Name：SP1;TSFP1;Transcription factor Sp1
Observed Band(KD)：81kD
Background： The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],
Function：function:Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.,PTM:O-glycosylated; contains N-acetylglucosamine side chains.,similarity:Belongs to the Sp1 C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subunit:Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr. Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the two proteins in DNA binding.,
Subcellular Location：Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
Expression：Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels.
