Target:SPAG5
Gene Name:SPAG5
Protein Name:Sperm-associated antigen 5 (Astrin) (Deepest) (Mitotic spindle-associated protein p126) (MAP126)
Human Gene Id:10615
Human Swiss Prot No:Q96R06
Mouse Swiss Prot No:Q7TME2
Immunogen:Synthesized peptide derived from part region of human protein
Specificity:SPAG5 Polyclonal Antibody detects endogenous levels of protein.
Formulation:Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Source:Polyclonal, Rabbit,IgG
Dilution:WB 1:500-2000 ELISA 1:5000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-15°C to -25°C/1 year(Do not lower than -25°C)
Observed Band(KD):131kD
Background: This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008],
Function:function:Necessary for normal spindle formation during mitosis.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,subcellular location:In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed.,subunit:Homodimer, with a globular head domain and a long stalk. Homo-oligomer; the globular head domains associate, resulting in aster-like structures. Binds to microtubules in the mitotic spindle.,tissue specificity:Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.,
Subcellular Location:Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . Chromosome, centromere, kinetochore . Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Colocalizes with PCM1 at centriolar satellites throughout the cell cycle (PubMed:26297806). In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase (PubMed:27462074). Efficient targeting to the mitotic spindle may depend upon phosphorylation by GSK3B. Detected on kinetochores of chromosomes that have congress
Expression:Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.